Your Partner in Discovery: Customized Organoids for Breakthrough Science
About the Core
The overall goal of the ÌÇÐÄÊÓƵ of Medicine 3D Organoid Core is to develop state of the art advanced human tissue models and platforms for application to studies designed to improve current understanding of biology at epithelial surfaces. The core generates, maintains, and provides experimentally ready human donor tissue-derived organoid cultures customized for individual projects. In addition to providing developed models, the core is also focused on developing new tools, models, and platforms for the exploration of additional aspects of epithelial biology. Centralized services provided by the core control and minimize costs, ensure quality control, and allow novel biological insight to be sought by the user. The core allows for the optimization and customization of core reagents by the user in consideration of unique aspects of individual projects.
Unlock Advanced Research Capabilities with our 3D Organoid Core
- State-of-the-Art Organoid Models: Gain access to cutting-edge human ex vivo organoid models that serve as a crucial link between traditional animal models and clinical research, offering unparalleled relevance to human biology.
- Diverse and Inclusive Cohort: Our core offers a large collection of epithelial organoids, derived from human stem cells of donors with diverse demographic backgrounds. This diversity enhances the applicability and impact of your research findings.
- Experienced Team at Your Service: With over 13 years of expertise in organoid culturing, our experienced staff is available to support you at every stage—from experimental design to execution and data interpretation—ensuring you obtain high-quality data to propel your research forward.
- Centralized and Cost-Effective: By centralizing organoid activities within our core, we reduce your cost burden, decrease the time it takes to return data, and guarantee optimization and stringent quality control throughout your project.
- Tailored Scientific Collaboration: Your research questions are our priority. We offer personalized interactions where your scientific inquiries are seamlessly integrated into the use of our organoid models, ensuring a collaborative and effective research experience.
Director's Message
As director of the BCM 3D Organoid Core, I am excited to help users integrate this technology into their research programs. Organoids are cost effective, high throughput, and provide a human model of epithelial surfaces. Technology such as human organoids has recently been recognized by the FDA Modernization Act 2.0 to represent an animal free alternative to develop biological products for human health and these models may have better predictive power than traditional animal models or transformed cell lines. My vision is to create human based platforms that can provide personalized medicine-based approaches to health care to improve clinical outcomes at the individual level. The mission of the core is to help users discover, develop, and implement organoid technology in their research programs studying human health and disease. I invite you to browse our website and imagine how organoid technology can transform your biological questions.
Publications
2024
Adeniyi-Ipadeola GO, Hankins JD, Kambal A, Zeng X-L, Patil K, Poplaski V, Bomidi C, Nguyen-Phuc H, Grimm SL, Coarfa C, Stossi F, Crawford SE, Blutt SE, Speer AL, Estes MK, Ramani S. . mBio. 2024 Aug 14;15(8):e0131624. doi: 10.1128/mbio.01316-24. Epub 2024 Jul 2. PMID: 38953637; PMCID: PMC11323560.
2023
Poplaski V, Bomidi C, Kambal A, Nguyen-Phuc H, Di Rienzi SC, Danhof HA, Zeng XL, Feagins LA, Deng N, Vilar E, McAllister F, Coarfa C, Min S, Kim HJ, Shukla R, Britton R, Estes MK, Blutt SE. . J Clin Invest. 2023 Nov 1;133(21):e166884. doi: 10.1172/JCI166884. PMID: 37909332; PMCID: PMC10617781.
2021
Rajan A, Weaver AM, Aloisio GM, Jelinski J, Johnson HL, Venable SF, McBride T, Aideyan L, Piedra FA, Ye X, Melicoff-Portillo E, Yerramilli MRK, Zeng XL, Mancini MA, Stossi F, Maresso AW, Kotkar SA, Estes MK, Blutt S, Avadhanula V, Piedra PA. . mBio. 2021 Feb 22;13(1):e0351121. doi: 10.1128/mbio.03511-21. Epub 2022 Feb 15. PMID: 35164569; PMCID: PMC8844923.
Criss ZK 2nd, Bhasin N, Di Rienzi SC, Rajan A, Deans-Fielder K, Swaminathan G, Kamyabi N, Zeng XL, Doddapaneni H, Menon VK, Chakravarti D, Estrella C, Yu X, Patil K, Petrosino JF, Fleet JC, Verzi MP, Christakos S, Helmrath MA, Arimura S, DePinho RA, Britton RA, Maresso AW, Grande-Allen KJ, Blutt SE, Crawford SE, Estes MK, Ramani S, Shroyer NF. . Physiol Genomics. 2021 Nov 1;53(11):486-508. doi: 10.1152/physiolgenomics.00061.2021. Epub 2021 Oct 6. PMID: 34612061; PMCID: PMC8616596.
Blutt SE, Crawford SE, Bomidi C, Zeng XL, Broughman JR, Robertson M, Coarfa C, Tessier MEM, Savidge T, Hollinger FB, Curley SA, Donowitz M, Estes MK. . Am J Physiol Gastrointest Liver Physiol. 2021 Sep 1;321(3):G270-G279. doi: 10.1152/ajpgi.00177.2021. Epub 2021 Jul 21. PMID: 34288725; PMCID: PMC8461792.
Wilson RL, Swaminathan G, Ettayebi K, Bomidi C, Zeng XL, Blutt SE, Estes MK, Grande-Allen KJ. . Tissue Eng Part C Methods. 2021 Jan;27(1):12-23. doi: 10.1089/ten.TEC.2020.0306. PMID: 33334213; PMCID: PMC7826425.
Hasan NM, Johnson KF, Yin J, Baetz NW, Fayad L, Sherman V, Blutt SE, Estes MK, Kumbhari V, Zachos NC, Kovbasnjuk O. . Mol Metab. 2021 Feb;44:101129. doi: 10.1016/j.molmet.2020.101129. Epub 2020 Nov 25. PMID: 33246140; PMCID: PMC7770968.
2020
In JG, Yin J, Atanga R, Doucet M, Cole RN, DeVine L, Donowitz M, Zachos NC, Blutt SE, Estes MK, Kovbasnjuk O. . iScience. 2020 Sep 28;23(10):101618. doi: 10.1016/j.isci.2020.101618. PMID: 33089106; PMCID: PMC7559866.
Rajan A, Robertson MJ, Carter HE, Poole NM, Clark JR, Green SI, Criss ZK, Zhao B, Karandikar U, Xing Y, Margalef-Català M, Jain N, Wilson RL, Bai F, Hyser JM, Petrosino J, Shroyer NF, Blutt SE, Coarfa C, Song X, Prasad BV, Amieva MR, Grande-Allen J, Estes MK, Okhuysen PC, Maresso AW. . PLoS Pathog. 2020 Sep 28;16(9):e1008851. doi: 10.1371/journal.ppat.1008851. PMID: 32986782; PMCID: PMC7553275.
Chakravarti D, Hu B, Mao X, Rashid A, Li J, Li J, Liao WT, Whitley EM, Dey P, Hou P, LaBella KA, Chang A, Wang G, Spring DJ, Deng P, Zhao D, Liang X, Lan Z, Lin Y, Sarkar S, Terranova C, Deribe YL, Blutt SE, Okhuysen P, Zhang J, Vilar E, Nielsen OH, Dupont A, Younes M, Patel KR, Shroyer NF, Rai K, Estes MK, Wang YA, Bertuch AA, DePinho RA. . Nat Commun. 2020 Sep 21;11(1):4766. doi: 10.1038/s41467-020-18420-w. PMID: 32958778; PMCID: PMC7505960.
2019
Fasciano AC, Blutt SE, Estes MK, Mecsas J. . J Vis Exp. 2019 Jul 26;(149):10.3791/59894. doi: 10.3791/59894. PMID: 31403623; PMCID: PMC6755909.
2017
Vernetti L, Gough A, Baetz N, Blutt S, Broughman JR, Brown JA, Foulke-Abel J, Hasan N, In J, Kelly E, Kovbasnjuk O, Repper J, Senutovitch N, Stabb J, Yeung C, Zachos NC, Donowitz M, Estes M, Himmelfarb J, Truskey G, Wikswo JP, Taylor DL. . Sci Rep. 2017 Feb 8;7:42296. doi: 10.1038/srep42296. Erratum in: Sci Rep. 2017 Mar 16;7:44517. doi: 10.1038/srep44517. PMID: 28176881; PMCID: PMC5296733.
2016
Ettayebi K, Crawford SE, Murakami K, Broughman JR, Karandikar U, Tenge VR, Neill FH, Blutt SE, Zeng XL, Qu L, Kou B, Opekun AR, Burrin D, Graham DY, Ramani S, Atmar RL, Estes MK. . Science. 2016 Sep 23;353(6306):1387-1393. doi: 10.1126/science.aaf5211. Epub 2016 Aug 25. PMID: 27562956; PMCID: PMC5305121.
2015
Saxena K, Blutt SE, Ettayebi K, Zeng XL, Broughman JR, Crawford SE, Karandikar UC, Sastri NP, Conner ME, Opekun AR, Graham DY, Qureshi W, Sherman V, Foulke-Abel J, In J, Kovbasnjuk O, Zachos NC, Donowitz M, Estes MK. . J Virol. 2015 Oct 7;90(1):43-56. doi: 10.1128/JVI.01930-15. PMID: 26446608; PMCID: PMC4702582.